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[Filtration of buffy coat-free erythrocyte suspensions in additive solution]
K Dietrich1, G Dietrich, V Kretschmer
1Klinikum Marburg, Abteilung Transfusionsmedizin und Gerinnungsphysiologie, BRD.
Insights
Filtration of buffy coat-free red cell concentrates (RCC) using Sepacell R 500 B filters showed effective leukocyte reduction with acceptable hemolysis and red cell loss. Bedside filtration is not recommended due to quality assurance needs.
Area of Science:
- Blood banking and transfusion medicine
- Biomedical engineering
- Hematology
Context:
- Buffy coat-free red cell concentrates (RCC) in SAG-M are stored for 4 weeks.
- Filtration is performed using Erypur Optima (E), Sepacell R 500 B (S), and PALL RC 50 TM (P) filters.
- Leukocyte content in RCC initially is 132 x 10^6/RCC.
Purpose:
- To evaluate the efficacy of different filters (E, S, P) for leukocyte reduction in stored buffy coat-free RCC.
- To assess the impact of filtration on hemolysis and red cell loss.
- To determine optimal filtration parameters and filter suitability for RCC preparation.
Summary:
- Leukocyte depletion was highly effective across all filters (E: 0.56, S: 0.36, P: 0.55 x 10^6/RCC).
- Hemolysis was significant, particularly with filters E (301 +/- 195 mg/RCC) and P (368 +/- 256 mg/RCC), while filter S showed lower hemolysis (127 +/- 123 mg/RCC).
- Filter S allowed preparation of two RCCs per filter with acceptable hemolysis and tolerable red cell loss (41.8%). Filters E and P showed higher red cell loss (90.5% and 38.2% respectively). Filtration times were shortest for E (6.6 min) and S (4.7 min) without added pressure.
Impact:
- The use of buffy coat-free RCC in additive solution mitigates filtration challenges, including storage interval, leukocyte reduction, hemolysis, and filtration flow.
- Filter S is the most suitable for preparing RCC, allowing two units per filter with acceptable outcomes.
- Despite filtration improvements, bedside filtration is not advised due to the necessity of rigorous quality assurance.
Abstract:
Buffy coat-free red cell concentrates in SAG-M (RCC) were produced by BAT system (leukocyte content 132 x 10(6)/RCC). They were stored for 4 weeks and filtered by Erypur Optima (E), Sepacell R 500 B (S) und PALL RC 50 TM (P). Leukocyte depletion was very effective (E: 0.56 x 10(6)/RCC; S: 0.36 x 10(6)/RCC; P: 0.55 x 10(6)/RCC) but hemolysis was remarkable (E: 301 +/- 195; S: 127 +/- 123; P: 368 +/- 256 mg/RCC). Therefore preparation of two RCC per filter was only acceptable with S. In S and P loss of red cells was tolerable (E: 90.5; S. 41.8; P: 38.2). In contrast, E should be rinsed with sodium chloride at the end of the preparation. In E and S filtration times were short without additional pressure (E: 6.6 min; S: 4.7 min; P: 20.3 min). We conclude from our results that the use of buffy coat-free red cell concentrates in additive solution considerably reduces the problems of filtration, e.g. storage interval, leukocyte reduction, hemolysis, filtration flow. Despite this, bedside filtration is not recommended because quality assurance is necessary.