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Detection of antibodies to HCV: comparison of a c100-3 EIA and the 2nd generation EIA
S Polywka1, P Kaars-Wiele, E Schroeter
1Institute of Medical Microbiology and Immunology, University Hospital Eppendorf, Hamburg, FRG.
The reactivity of 558 serum specimens in Abbott 1st and 2nd generation EIAs was compared. A significant increase in HCV seropositivity was especially found in high risk groups such as hemophiliacs (57.7 vs. 73.1%), IVDA (54.5 vs. 61.8%), HIV positives (21.8 vs. 25.5%) or patients on dialysis (15.7 vs. 16.9%). Reactivity of all sera which only reacted in the 2nd generation EIA was 'confirmed' by using supplemental research assays. In contrast, sera which were highly reactive in the c100-3 assay but could not be confirmed by Neutralization Assay were weakly positive or negative in the 2nd generation test.
The reactivity of 558 serum specimens in Abbott 1st and 2nd generation EIAs was compared. A significant increase in HCV seropositivity was especially found in high risk groups such as hemophiliacs (57.7 vs. 73.1%), IVDA (54.5 vs. 61.8%), HIV positives (21.8 vs. 25.5%) or patients on dialysis (15.7 vs. 16.9%). Reactivity of all sera which only reacted in the 2nd generation EIA was 'confirmed' by using supplemental research assays. In contrast, sera which were highly reactive in the c100-3 assay but could not be confirmed by Neutralization Assay were weakly positive or negative in the 2nd generation test.