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Updated: Aug 18, 2026

Analysis of Cell Cycle Position in Mammalian Cells
Published on: January 21, 2012
[The influence of paraquat on cell cycle--analysis of cell kinetics using DNA/BrdU staining]
M Matsubara1, M Sakai, K Yamagami
1Department of Emergency and Critical Care Medicine, Kansai Medical University, Japan.
Abstract:
We examined the in vitro and in vivo effects of paraquat on the cell cycle. After we exposed paraquat to the cultured cell (MDCK), we examined cell kinetics by flow cytometry with BrdU-PI double staining. And we administered paraquat intravenously to the rats, we collected and separated rat's liver and pulmonary cells, and we examined the cell kinetics by same method. Consequently our study found that the cells in the S-phase were noted to be abundant, and we suggested that paraquat apparently arrested the cell cycle at S time, and that DNA damage was importance in the mechanism of paraquat toxicity.
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