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Related Experiment Videos

Solid phase microextraction-gas chromatography for quantifying headspace hexanal above freeze-dried chicken

Carolyn F Goodridge1, Randolph M Beaudry, James J Pestka

  • 1Department of Food Science and Human Nutrition, Michigan State University, East Lansing, Michigan 48824, USA. cgoodrid@scimail.uwarterloo.ca

Journal of Agricultural and Food Chemistry
|July 10, 2003
PubMed
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This study developed a rapid solid phase microextraction (SPME) method with gas chromatography/mass spectrometry (GC/MS) to detect lipid oxidation in chicken. This technique accurately measured hexanal, a key oxidation indicator, in freeze-dried chicken myofibrils.

Area of Science:

  • Food Science
  • Analytical Chemistry
  • Biochemistry

Background:

  • Lipid oxidation in food products like chicken leads to undesirable changes in quality and nutritional value.
  • Freeze-dried chicken myofibrils can act as a reservoir for volatile compounds, influencing oxidation pathways.
  • Accurate and rapid methods are needed to quantify lipid oxidation indicators.

Purpose of the Study:

  • To develop and validate a solid phase microextraction (SPME) combined with gas chromatography/mass spectrometry (GC/MS) method for determining lipid oxidation in freeze-dried chicken myofibrils.
  • To assess the influence of storage time and water activity on lipid oxidation.
  • To compare the developed SPME-GC/MS method with traditional assays like TBARS.

Main Methods:

  • Solid phase microextraction (SPME) coupled with gas chromatography/mass spectrometry (GC/MS) was employed.

Related Experiment Videos

  • Headspace hexanal concentration was measured as an indicator of lipid oxidation.
  • Thiobarbituric acid reactive substances (TBARS) assay and conjugated diene assay were used for comparison.

    • Main Results:

    • The SPME-GC/MS method demonstrated high recovery (95%) and a rapid analysis time (12 min/sample).
    • A strong linear response (r² = 0.995) was observed for hexanal quantification.
    • Lipid oxidation was significantly influenced by storage time and water activity, with a strong correlation (r = 0.938) between SPME-GC/MS and TBARS results.

    Conclusions:

    • SPME-GC/MS is a sensitive, rapid, and reliable method for detecting hexanal as a marker of lipid oxidation in chicken myofibrils.
    • The method allows for effective monitoring of oxidation under various storage conditions.
    • This technique offers a valuable tool for quality control in the food industry.