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Effects of temperature and host cell type on the in vitro growth and development of Sarcocystis falcatula

Hany M Elsheikha1, Mahdi A Saeed, Scott D Fitzgerald

  • 1Molecular Epidemiology Laboratory, National Food Safety and Toxicology Center, Michigan State University, East Lansing, MI 48824, USA.

Parasitology Research
|July 10, 2003
PubMed

Insights

Sarcocystis falcatula grows best in Vero and bovine turbinate cells compared to other cell lines. Incubation temperature did not significantly impact parasite growth in vitro.

Area of Science:

  • Parasitology
  • Cell Biology
  • Infectious Diseases

Background:

  • Sarcocystis falcatula is an intracellular protozoan parasite.
  • Understanding its in vitro growth is crucial for developing control strategies.

Purpose of the Study:

  • To evaluate the in vitro growth and proliferation of Sarcocystis falcatula in various cultured cell lines.
  • To determine the optimal cell line and temperature for S. falcatula culture.

Main Methods:

  • Cultured merozoites of S. falcatula were inoculated into Vero, bovine turbinate (BT), equine dermal (ED), and human Hep-2 cell lines.
  • Parasite yield, viability, and plaque-forming efficiency were assessed.
  • Experiments were conducted at two incubation temperatures: 35°C and 37°C.

Main Results:

  • Vero and BT cells demonstrated superior support for S. falcatula growth compared to ED and Hep-2 cells.
  • Mean parasite yields per T25 flask after 18 days: Vero (1.3x10^7), BT (1.1x10^7), ED (0.9x10^7), and Hep-2 (0.7x10^7).
  • Incubation temperature (35°C vs. 37°C) did not significantly affect parasite growth across all tested cell lines.

Conclusions:

  • The choice of host cell line significantly influences the in vitro growth of S. falcatula.
  • Vero and BT cells are recommended for optimal in vitro cultivation of S. falcatula.
  • Temperature is a less critical factor than host cell type for S. falcatula proliferation in vitro.

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