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Screening method for detecting cross-contamination residues of tiamulin in swine feeds
Mirian Rodríguez-Comesaña1, Beatriz Cancho-Grande, Jesús Simal-Gándara
1University of Vigo, Analytical and Food Chemistry Department, Faculty of Food Science and Technology, Nutrition and Bromatology Group, Ourense Campus, 32004 Ourense, Spain.
Abstract:
A method was developed for the determination of tiamulin (TML), 14-deoxy-14-[(2-diethylaminoethyl)mercaptoacetoxy]mutilin hydrogen fumarate, a semisynthetic derivative of the naturally occurring antibiotic pleuromutilin produced by the fungus Pleurotus mutilis. This drug, with high activity against Gram-positive bacteria, some Gram-negative bacteria, and several strains of mycoplasms is administered to animals in food, drinking water, or by injection; however, its chemical structure causes problems in analysis of feeds. Although the molecule is charged below pH 8, attempts to analyze TML-containing extracts on ion-exchange columns or other polar stationary phases have failed. Additionally, TML shows no fluorescence activity and only poor UV activity. The present method consists of organic solvent extraction followed by liquid chromatography with UV detection. A low wavelength (208 nm) was used for detection. Limits of detection and quantitation, as well as data for recovery and repeatability obtained during characterization of the method, are described. The applicability of the optimized method was tested by analyzing commercial blank feeds processed after TML-medicated feeds.
Insights
A new method accurately determines tiamulin (TML) in animal feeds. This liquid chromatography technique overcomes previous analytical challenges for this important veterinary antibiotic.
Area of Science:
- Veterinary Pharmacology
- Analytical Chemistry
- Food Safety
Background:
- Tiamulin (TML) is a vital antibiotic for animal health, derived from pleuromutilin.
- Its chemical properties present significant challenges for accurate feed analysis.
- Existing analytical methods are insufficient for TML determination in complex matrices.
Purpose of the Study:
- To develop and validate a robust analytical method for tiamulin (TML) determination in animal feed.
- To overcome the limitations of previous analytical approaches for TML.
- To ensure accurate quantification of TML in feed for regulatory compliance and animal safety.
Main Methods:
- Organic solvent extraction of tiamulin (TML) from feed samples.
- High-performance liquid chromatography (HPLC) with UV detection at a low wavelength (208 nm).
- Method validation including limits of detection, quantitation, recovery, and repeatability.
Main Results:
- Successful development of a sensitive and specific method for TML analysis.
- Demonstrated high recovery and repeatability of the method.
- Validated the method's applicability by analyzing commercial blank feeds post-medication.
Conclusions:
- The developed method provides a reliable approach for tiamulin (TML) quantification in animal feeds.
- This method addresses the analytical difficulties previously associated with TML.
- Enables accurate monitoring of TML residues in feed, supporting animal health and food safety.