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Clay-based affinity probes for selective cleanup and determination of aflatoxin B1 using nanostructured
Henry J Huebner1, Timothy D Phillips
1Texas A&M University, College of Veterinary Medicine, Department of Veterinary Anatomy and Public Health, Faculty of Toxicology, College Station, TX 77843-4458, USA.
Abstract:
A study was conducted to investigate the selective cleanup and determination of aflatoxin B1 (AfB1) from contaminated media. Composite adsorbents were formulated from calcium montmorillonite clay, which possesses a high affinity and enthalpy of adsorption for AfB1. Nanostructuring techniques were used to construct various formulations of the clay-based composite media. In AfB1 adsorption studies with prototypical affinity columns, these composites offered narrowly defined, reproducible capacity ranges. Composite recoveries of AfB1 from spiked grains exhibited linear trends that correlated well with the range of spike levels. Composite columns provided lower recoveries of AfB1 from naturally contaminated corn than did immunoaffinity columns; however, recoveries were consistent and purified extracts were free of interfering compounds, as determined by liquid chromatography with fluorescence detection.