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[Changes in the percentage of human leukocyte antigen DR in CD(+)(14) monocytes in burned patients]
Jing-jie Liu1, Sen Hu, Ning Dong
1Burn Institute, 304th Hospital of People's Liberation Army, Beijing 100037, China.
Objective:
To observe the changes in the percentage of CD(+)(14) monocytes expressing human leukocyte antigen DR(HLA-DR) in burn patients.
Methods:
Thirty burn patients were divided into three groups according to body surface area burned: severe burn group [>60% total body surface area(TBSA)]; moderate burn group (25%-30% TBSA), mild burn group(<10% TBSA). Blood samples were collected on 3, 7, 14, 28 and 49 postburn days(PBD), and CD(+)(14) monocyte HLA-DR expression was determined with monoclonal antibody staining and flow cytometry.
Results:
After burn injury, the percentage of monocytes expressing HLA-DR in mild burned patients was (58.80+/-5.60)%. The monocyte HLA-DR level was significantly reduced to below 30% on 3 PBD in both severely burned patients and moderately burned patients[(4.30+/-1.50)% and (13.86+/-2.40)%] respectively. The level of monocyte HLA-DR in moderately burned patients recovered on 21 PBD to above 30% while that of severely burned patients continued to be lowered till discharge from the hospital. There were statistical significant differences in HLA-DR expression between severely burned patients and moderately burned patients on 3, 7 and 14 PBD.
Conclusion:
The results suggested that immunity paralysis lasted for a long time in severely burned patients, and the percentage of monocytes expressing HLA-DR could be an important index in evaluating immune function of burn patients. Therefore, therapy to enhance immunity may be beneficial in the treatment of burn patients.
