Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Getting (chromosomes) loaded--a new role for timeless.

Andy Golden1, Orna Cohen-Fix

  • 1Laboratory of Biochemistry and Genetics, NIDDK, NIH, Bethesda, MD 20892, USA.

Developmental Cell
|July 11, 2003
PubMed
Summary

A new study in C. elegans links the TIM-1 protein to sister chromatid cohesion, suggesting a stepwise loading of cohesin. This finding may also impact understanding of circadian rhythms.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Elevated body temperature exacerbates arrhythmia and seizure-like activity in a zebrafish model of Timothy syndrome.

Communications biology·2026
Same author

The centriculum, a membrane reticulum that surrounds Caenorhabditis elegans centrosomes, might serve as a microtubule filter.

Journal of cell science·2026
Same author

Kar4 acts as a Ste12 regulator in Saccharomyces cerevisiae, promoting Ste12 binding to a specific DNA motif genome-wide.

Genetics·2026
Same author

Kar4 acts as a Ste12 regulator in <i>Saccharomyces cerevisiae</i>, promoting Ste12 binding to a specific DNA motif genome-wide.

bioRxiv : the preprint server for biology·2025
Same author

The centriculum, a membrane that surrounds <i>C. elegans</i> centrosomes, acts as a microtubule filter.

bioRxiv : the preprint server for biology·2025
Same author

Elevated body temperature exacerbates arrhythmia and seizure-like activity in a zebrafish model of Timothy syndrome.

bioRxiv : the preprint server for biology·2025

Area of Science:

  • Genetics
  • Cell Biology
  • Chronobiology

Background:

  • Sister chromatid cohesion is crucial for accurate chromosome segregation during cell division.
  • The TIM-1 protein in C. elegans is a homolog of Drosophila TIMELESS, known for its role in circadian rhythms.
  • The precise molecular mechanisms governing cohesin loading onto chromosomes remain incompletely understood.

Purpose of the Study:

  • To investigate the function of the TIM-1 protein in C. elegans.
  • To explore a potential novel role for TIM-1 in sister chromatid cohesion.
  • To determine if TIM-1 influences the stepwise loading of cohesin subunits.

Main Methods:

  • Analysis of phenotypic data from tim-1 mutant strains in C. elegans.
  • Comparative analysis with known functions of TIMELESS homologs in other species.
  • Genetic and molecular assays to assess cohesin loading dynamics.

Main Results:

  • Mutant phenotypes of tim-1 suggest a role in sister chromatid cohesion.
  • Evidence indicates that cohesin subunits may load onto chromosomes in a stepwise manner mediated by TIM-1.
  • The study discusses the potential involvement of TIM-1 in regulating circadian rhythms.

Conclusions:

  • TIM-1 plays an unexpected role in sister chromatid cohesion in C. elegans.
  • The findings propose a model for stepwise cohesin subunit loading.
  • Further research is warranted to elucidate TIM-1's function in both cell division and circadian biology.

Related Experiment Videos