Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

REDOR with a relative full-echo reference.

Anil K Mehta1, Lynette Cegelski, Robert D O'Connor

  • 1Department of Chemistry, Washington University, One Brookings Dr., Campus Box 1134, St. Louis, MO 63130, USA.

Journal of Magnetic Resonance (San Diego, Calif. : 1997)
|July 11, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cross-Molecular Active Learning for the Discovery of Antimicrobial Polyacrylamides.

bioRxiv : the preprint server for biology·2025
Same author

Three-dimensional navigation for talocalcaneal coalition resection: A comparative analysis.

The Journal of foot and ankle surgery : official publication of the American College of Foot and Ankle Surgeons·2025
Same author

Glycogen phase-separation drives macromolecular rearrangement and asymmetric division in E. coli.

The EMBO journal·2025
Same author

Structure and Biosynthesis of Aridomycins Reveal a Glycosylated Prodrug Strategy for Self-Resistance.

Journal of the American Chemical Society·2025
Same author

Polyacrylamide-Based Antimicrobial Copolymers to Replace or Rescue Antibiotics.

ACS central science·2025
Same author

A Systematic Approach to Discover New Natural Product Scaffolds Using Database-Derived Relative Mass Spectral Defects and Molecular Networking.

JACS Au·2025
Same journal

Localization-driven exchange contrast in diffusion exchange spectroscopy.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2026
Same journal

4.5 Tesla superconducting miniature magnet in liquid nitrogen.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2026
Same journal

Folding and unfolding dynamics of a DNA aptamer studied by heteronuclear <sup>1</sup>H-<sup>13</sup>C correlation zz-exchange spectroscopy.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2026
Same journal

Multi-spin control from one-spin pulses.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2026
Same journal

Altering MRI rotating frame relaxations by changing the truncation level of Hyperbolic Secant pulse.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2026
Same journal

Effects of proton exchange on the lifetimes of long-lived states in aliphatic chains.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2026
See all related articles

This study used advanced NMR techniques to map the binding site of a vancomycin-like antibiotic within Staphylococcus aureus cell walls. Researchers precisely located the drug

Area of Science:

  • Biochemistry
  • Microbiology
  • Structural Biology

Background:

  • Staphylococcus aureus is a significant human pathogen.
  • Vancomycin is a critical antibiotic for treating S. aureus infections.
  • Understanding antibiotic-cell wall interactions is crucial for developing new therapeutics.

Purpose of the Study:

  • To determine the precise binding site of a vancomycin analogue within the Staphylococcus aureus cell wall.
  • To investigate the structural basis of antibiotic-peptidoglycan interactions.

Main Methods:

  • Solid-state Nuclear Magnetic Resonance (NMR) spectroscopy, specifically Rotational Echo DOuble Resonance (REDOR) and REDOR-like experiments.
  • Utilized 13C[19F] and 2H[19F] NMR on whole bacterial cells.
  • Incorporated isotopically labeled amino acids (L-[13C(3)]alanine or L-[methyl-d(3)]alanine) into bacterial peptidoglycan.

Related Experiment Videos

Main Results:

  • The 19F atom of the vancomycin analogue was localized relative to 13C and 2H labels in the peptidoglycan peptide stem.
  • The fluorinated biphenyl moiety of the drug was found to be in close proximity to specific residues of the peptidoglycan.
  • Dipolar recoupling methods allowed for precise distance measurements without requiring absolute echo measurements.

Conclusions:

  • The study provides high-resolution structural information on antibiotic binding to bacterial cell walls.
  • This detailed mapping can inform the design of novel antibiotics with improved efficacy and reduced resistance.
  • Solid-state NMR is a powerful tool for elucidating drug-target interactions in complex biological systems.