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Rotational-echo double resonance of uniformly labeled 13C clusters
1Department of Chemistry, Washington University, One Brookings Drive, Campus Box 1134, St. Louis, MO 63130-4899, USA.
Journal of Magnetic Resonance (San Diego, Calif. : 1997)
|July 11, 2003
Abstract:
The use of rotational-echo double resonance NMR to measure distances from an observed tightly coupled cluster of 13C spins to a distant 15N, 31P, or 19F is practical if 13C chemical shifts and homonuclear 13C-13C isotropic J interactions are refocused by a combination of rotor-synchronized 13C pi and pi/2 pulses. This scheme is illustrated by experiments performed on diluted and recrystallized L-[13C(3),15N]alanine and L-[13C(6),alpha-15N]histidine.