Related Experiment Video
Updated: Aug 24, 2026

Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules
Published on: June 1, 2011
Molecular probe for selective detection of thiols in water of neutral pH
1Center for Integrated Molecular Systems and Department of Chemistry, Division of Molecular and Life Sciences, Pohang University of Science and Technology, San 31 Hyoja-dong, Pohang 790-784, South Korea. dhkim@postech.ac.kr
Abstract:
The ternary complex, [Zn(cyclen)(lumazine)](+) that can be readily obtained by mixing cyclen, zinc perchlorate, and lumazine in water in a 1:1:1 molar ratio serves as a molecular probe that detects selectively thiols in an aqueous solution of neutral pH. Thiols successfully displace the lumazine in the molecular probe, which is accompanied by a decrease of the fluorescence. The molecular probe is useful for identification of cysteine among essential amino acids and detection of glutathione in aqueous solution of neutral pH.
Related Concept Videos
Labeling DNA Probes
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Preparation and Reactions of Thiols
Precipitation Titration: Endpoint Detection Methods
In the Volhard method, a standard excess of AgNO3 is first added to the...

