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Multi-Scale Modification of Metallic Implants With Pore Gradients, Polyelectrolytes and Their Indirect Monitoring In vivo
Published on: July 1, 2013
IL-6 and PGE2 release by human osteoblasts on implant materials
C Schmidt1, G Steinbach, R Decking
1Institute of Orthopaedic Research and Biomechanics, RKU University of Ulm, Helmholtzstr. 14, 89081, Ulm, Germany. carla.schmidt@medizin.uni-ulm.de
Biomaterials
|July 11, 2003
Summary
Orthopaedic implant materials did not significantly affect the release of interleukin-6 (IL-6) and prostaglandin E(2) (PGE(2)) from human osteoblasts. The released mediators were likely insufficient to cause bone resorption and implant loosening.
Area of Science:
- Biomaterials Science
- Orthopaedic Surgery
- Immunology
Background:
- Orthopaedic implant loosening is a significant clinical challenge.
- Particle-induced inflammation, involving macrophages and osteoblasts, is hypothesized to cause bone resorption and implant failure.
- Key mediators implicated include interleukin-1 (IL-1), tumor necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6), and prostaglandin E(2) (PGE(2)).
Purpose of the Study:
- To compare the in vitro ability of clinically relevant orthopaedic implant materials to induce IL-6 and PGE(2) release from human osteoblasts.
- To investigate whether material composition influences osteoblast mediator release independently of macrophage activation.
Main Methods:
- Primary human osteoblasts were cultured with stainless steel, cobalt-chromium alloy (CoCrMo), commercially pure titanium (cpTi), Ti-6Al-7Nb, and Ti-6Al-4V samples.
- Cells were also cultured with a plastic control.
- Supernatants were collected after 12 and 24 hours to measure IL-6 and PGE(2) concentrations.
Main Results:
- Osteoblasts actively secreted IL-6 and PGE(2) in response to all tested materials and the plastic control.
- No significant differences in IL-6 or PGE(2) release were observed among the different implant materials.
- The measured mediator levels were comparable to literature values for osteoblasts cultured on plastic and lower than levels induced by direct particle stimulation or inflammatory cytokines (IL-1, TNF-alpha).
Conclusions:
- The tested orthopaedic implant materials, with similar surface roughness, did not significantly influence IL-6 and PGE(2) release from human osteoblasts.
- The released concentrations of PGE(2) were likely insufficient to activate osteoclasts and induce osteolysis.
- The role of the measured IL-6 concentrations in promoting osteolytic activity remains unclear due to conflicting literature.
- Material composition differences did not significantly impact mediator release under these experimental conditions.
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