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Enzyme-linked immunosorbent assay for quantitation of human antibodies to pneumococcal polysaccharides

Catherine M Wernette1, Carl E Frasch, Dace Madore

  • 1Department of Pathology and Microbiology, University of Alabama at Birmingham, Birmingham, Alabama 35249-7331, USA.

Clinical and Diagnostic Laboratory Immunology
|July 11, 2003
PubMed
Abstract

No abstract available in PubMed .

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Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.