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Vertebrate HoxB gene expression requires DNA replication.

Daniel Fisher1, Marcel Méchali

  • 1Institute of Human Genetics, CNRS, 141 rue de la Cardonille, 34396 Cedex 05 Montpellier, France.

The EMBO Journal
|July 11, 2003
PubMed
Summary

Hox gene activation during development is dependent on DNA replication. This process requires S phase and occurs within a single cell cycle, suggesting replication remodels the locus for transcription.

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Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Genetics

Background:

  • DNA replication and gene transcription are fundamental cellular processes.
  • Understanding their interplay is crucial for comprehending gene regulation during development.
  • Hox genes play a critical role in establishing body plan and development.

Purpose of the Study:

  • To investigate the relationship between DNA replication and Hox gene transcription in vivo.
  • To determine the role of cell cycles and DNA replication in HoxB gene activation.
  • To elucidate the timing and requirements of HoxB locus activation during vertebrate development.

Main Methods:

  • Studied Hox gene activation in Xenopus embryogenesis and mouse P19 cell differentiation.
  • Utilized synchronous cell cultures to analyze gene activation within a single cell cycle.

Related Experiment Videos

  • Investigated the correlation between DNA replication (S phase) and HoxB gene expression patterns.

    • Main Results:

    • HoxB activation in Xenopus requires early cell cycles post-mid-blastula transition.
    • HoxB expression in P19 cells is proliferation-dependent and occurs within one cell cycle.
    • HoxB gene activation is colinear, occurs during S phase, and is DNA replication-dependent.
    • The HoxB locus replicates early, but replication remains necessary for maximal expression later in S phase.

    Conclusions:

    • Hox gene induction is dependent on DNA replication.
    • Activation of the HoxB locus is licensed for transcriptional regulation by S-phase-dependent chromatin remodeling.
    • A single cell cycle, coupled with DNA replication, is sufficient for HoxB gene activation.