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Updated: Sep 23, 2026
Analysis of Yersinia enterocolitica Effector Translocation into Host Cells Using Beta-lactamase Effector Fusions
Published on: October 13, 2015
Comparative analysis of type III effector translocation by Yersinia pseudotuberculosis expressing native LcrV or PcrV
Jeanette E Bröms1, Charlotta Sundin, Matthew S Francis
1Department of Molecular Biology, Umeå University, Umeå, Sweden.
Abstract:
The homologues LcrV of Yersinia species and PcrV of Pseudomonas aeruginosa are pore-forming components. When expressed in a Yersinia lcrV background, PcrV formed smaller pores in infected erythrocyte membranes, correlating to a lowered translocation of Yersinia effectors. To understand this phenomenon, cytotoxins exoenzyme S of P. aeruginosa and YopE of Yersinia were introduced into a Yersinia background without Yop effectors but expressing LcrV or PcrV. Comparable translocation of each substrate indicated that substrate recognition by LcrV/PcrV is not a regulator of translocation. Yersinia harboring pcrV coexpressed with its native operon efficiently translocated effectors into HeLa cell monolayers and formed large LcrV-like pores in erythrocyte membranes. Thus, a PcrV complex with native P. aeruginosa translocon components is required to form fully functional pores for complete complementation of effector translocation in Yersinia.
Insights
Pseudomonas aeruginosa PcrV forms smaller pores than Yersinia LcrV, reducing effector translocation. A complete PcrV complex with native components is necessary for functional pore formation and effector delivery in Yersinia.
Area of Science:
- Microbiology and Immunology
- Bacterial Pathogenesis
- Molecular Biology
Background:
- LcrV (Yersinia species) and PcrV (Pseudomonas aeruginosa) are homologous pore-forming proteins crucial for bacterial virulence.
- Differences in pore formation between LcrV and PcrV affect the translocation of bacterial effector proteins into host cells.
Purpose of the Study:
- To investigate the functional differences between LcrV and PcrV in Yersinia.
- To determine the role of native translocon components in PcrV-mediated pore formation and effector translocation.
Main Methods:
- Expressing PcrV in a Yersinia lcrV background and comparing pore size and effector translocation with native LcrV.
- Introducing P. aeruginosa exoenzyme S and Yersinia YopE into Yersinia strains expressing LcrV or PcrV.
- Analyzing effector translocation into HeLa cells and pore formation in erythrocyte membranes.
Main Results:
- PcrV in a Yersinia background formed smaller pores than LcrV, leading to reduced Yersinia effector translocation.
- Substrate recognition by LcrV/PcrV was not the primary regulator of effector translocation.
- Yersinia expressing pcrV along with its native operon components efficiently translocated effectors and formed large pores.
Conclusions:
- The PcrV complex, when reconstituted with native Pseudomonas aeruginosa translocon components, forms fully functional pores.
- This functional PcrV complex can complement effector translocation in Yersinia, highlighting the importance of the complete translocon structure.
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