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Construction and expression of recombined human AFP eukaryotic expression vector
Li-Wang Zhang1, Jun Ren, Liang Zhang
1Department of Oncology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, Shaanxi Province, China.
World Journal of Gastroenterology
|July 11, 2003
Summary
Researchers created a human AFP eukaryotic expression vector for hepatocellular carcinoma (HCC) gene therapy. The vector successfully expressed AFP antigen in CHO cells, offering data for HCC treatment strategies.
Area of Science:
- Molecular Biology
- Biotechnology
- Oncology
Background:
- Hepatocellular carcinoma (HCC) is a significant global health concern.
- Gene therapy and targeted therapy offer promising avenues for HCC treatment.
- Alpha-fetoprotein (AFP) is a biomarker and potential therapeutic target in HCC.
Purpose of the Study:
- To construct a functional human alpha-fetoprotein (AFP) eukaryotic expression vector.
- To evaluate the vector's utility for gene therapy and targeted therapy in hepatocellular carcinoma (HCC).
Main Methods:
- Full-length AFP-cDNA was isolated and subcloned into a eukaryotic expression vector.
- Vector construction was verified using enzyme digestion and electrophoresis.
- Expression of AFP was confirmed via electrochemiluminescence and immunofluorescence assays.
Main Results:
- Successful cloning of full-length AFP-cDNA into the eukaryotic vector was confirmed.
- Significant AFP antigen expression (0.9723 IU/ml) was detected in AFP-CHO cell supernatant.
- Immunofluorescence confirmed AFP antigen presence in the plasma of transfected cells.
Conclusions:
- The constructed human AFP eukaryotic expression vector is functional in CHO cells.
- This vector provides a valuable tool and experimental basis for HCC gene and targeted therapies.