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Related Experiment Videos

Caloric restriction in trans.

Bruce S Kristal1, Ugo Paolucci

  • 1Dementia Research Service, Burke Medical Research Institute, White Plains, NY 10605, USA. bkristal@burke.org

Science of Aging Knowledge Environment : SAGE KE
|July 12, 2003
PubMed
Summary

Dietary restriction (DR) extends longevity in mammals. This study shows DR effects can be reproduced in cell cultures using serum from restricted animals, offering a new model to study DR mechanisms.

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Area of Science:

  • Gerontology
  • Cell Biology
  • Biochemistry

Background:

  • Dietary restriction (DR) is a proven method for extending lifespan and reducing disease in mammals.
  • Key questions remain regarding DR's applicability to humans and its underlying biochemical mechanisms.

Purpose of the Study:

  • To investigate if the effects of dietary restriction (DR) can be replicated in mammalian tissue culture cells.
  • To develop a novel in vitro model for studying the trans-acting mechanisms of DR.

Main Methods:

  • Mammalian tissue culture cells were exposed to serum derived from rats and nonhuman primates subjected to dietary restriction (DR).
  • Cellular responses were analyzed to determine if DR-associated effects could be observed in vitro.

Main Results:

  • Serum from DR-treated animals induced CR-like effects in cultured mammalian cells.
  • This suggests that factors present in the serum mediate some of the beneficial effects of DR.

Conclusions:

  • Dietary restriction (DR) exerts some of its biological effects through circulating factors (in trans).
  • This study establishes a new cell culture model for dissecting the molecular mechanisms of DR.

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