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High-affinity oxygen uptake by Bifidobacterium bifidum.

R P Cox¹, N Marling

¹Institute of Biochemistry, Odense University, Denmark.

Antonie Van Leeuwenhoek

|November 1, 1992

Summary

Bifidobacterium bifidum DSM 20082 exhibits Michaelis-Menten kinetics for oxygen uptake, with a high affinity for oxygen demonstrated by its NADH oxidase activity. This study quantifies oxygen consumption rates in bacterial cell suspensions.

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Area of Science:

Microbiology
Biochemistry
Cellular Respiration

Background:

Bifidobacterium bifidum is a probiotic bacterium.
Understanding oxygen metabolism in anaerobic bacteria is crucial for their industrial applications and survival in the gut.
Previous studies on Bifidobacterium oxygen uptake are limited.

Purpose of the Study:

To investigate and quantify the oxygen uptake kinetics of Bifidobacterium bifidum DSM 20082.
To characterize the enzyme responsible for oxygen reduction in cell-free extracts.

Main Methods:

Spectrophotometric measurement of myoglobin oxygenation to determine dissolved oxygen concentration.
Computer analysis of absorbance changes to calculate oxygen consumption rates.
Enzyme assays on cell-free extracts to identify NADH oxidase activity.

Main Results:

Washed cell suspensions of Bifidobacterium bifidum DSM 20082 displayed Michaelis-Menten kinetics for oxygen uptake.
An apparent Km value of 0.06 microM O2 was determined, indicating high oxygen affinity.
Cell-free extracts revealed soluble NADH oxidase activity with high affinity for O2, reducing it to H2O.

Conclusions:

Bifidobacterium bifidum DSM 20082 possesses an efficient oxygen uptake system.
The identified NADH oxidase activity likely plays a key role in the bacterium's response to oxygen.
These findings contribute to understanding the respiratory metabolism of this important probiotic species.

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