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[Generation of recombinant human tissue factor pathway inhibitor in E. coli]
Xigang Leng1, Chuanhua Wang, Liping Song
1Institute of Biomedical Engineering, Chinese Academy of Medical Sciences & Peking Union Medical College, Bioactive Materials Key Lab of Ministry of Education, Nankai University, Tianjin.
Abstract:
Tissue factor pathway inhibitor (TFPI) is one of the major physiological inhibitors of the human blood coagulation cascade and may have great potential in the prevention and therapy of diseases caused by thrombus formation. In this study, recombinant human tissue factor was generated in E. coli containing a recombinant vector constructed by inserting TFPI cDNA into pGEX-2T vector. The generated recombinant TFPI (rTFPI) could be simply purified with glutathione-agarose affinity method and maintained its biological function in terms of inhibition of tissue factor and factor Xa.