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Preparation of Rat Skeletal Muscle Homogenates for Nitrate and Nitrite Measurements
Published on: July 29, 2021
[Relation between nitric oxide synthase, psychology, gastric mucosal blood flow and gastric mucosal adaptive
Shi-nan Nie1, Xiao-ming Qian, Wen-jie Tang
1Emergency Department, Nanjing General Hospital of Nanjing Command/Clinical School of Medical College of Nanjing University, Nanjing 210002, Jiangsu, China.
Objective:
To explore the relation between nitric oxide synthase (NOS), psychology, gastric mucosal blood flow(GMBF) and gastric mucosal adaptive cytoprotection under stress in rats and its possible mechanism.
Methods:
Animal model was constructed with impulsive stimulator of high voltage and stable currency, seventy-two male SD rats were randomly divided into three groups: control(C), regular(R) and irregular(I) group. NOS activity was measured by spectrophotometry and injure of gastric mucosa was measured by method of Nils Lambecht, GMBF was measured by LDF-3 flowmeter.
Results:
In both R group and I group, NOS increased significantly, but injury index(II) was on contrary apparently decreasing. GMBF was increased gradually after stress. In R group, there was a negative proportion between II and GMBF, in I group, there was a negative proportion between II and GMBF and NOS, but there was a positive relationship between NOS and GMBF.
Conclusion:
NOS, psychology and GMBF all might participate in the course of the gastric mucosal adaptive cytoprotection under stress.
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