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Related Concept Videos

DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
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DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart, a...
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The cell cycle is a series of events leading to DNA duplication followed by the division of cell content to form two daughter cells. The cell cycle progresses in four stages—the cell increases in size (gap 1 or G1-phase), duplicates its DNA (synthesis or S-phase), prepares to divide (gap 2 or G2-phase), and divides (mitosis or M-phase).
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of replication.
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Determination of S-Phase Duration Using 5-Ethynyl-2'-deoxyuridine Incorporation in Saccharomyces cerevisiae
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Schizosaccharomyces pombe Ddb1 is functionally linked to the replication checkpoint pathway.

Tanya Bondar1, Ekaterina V Mirkin, David S Ucker

  • 1Department of Biochemistry, University of Illinois, Chicago, Illinois 60612, USA.

The Journal of Biological Chemistry
|July 15, 2003
PubMed
Summary

Schizosaccharomyces pombe Ddb1 protein is linked to cell division and chromosomal segregation. Deleting ddb1 causes replication defects and aberrant Cds1 kinase activity, suggesting Ddb1

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Area of Science:

  • Cell biology
  • Molecular genetics
  • DNA repair

Background:

  • Schizosaccharomyces pombe Ddb1 protein is homologous to mammalian DDB1, involved in DNA repair.
  • Recent studies suggest S. pombe Ddb1 plays a role in cell division and chromosomal segregation.

Purpose of the Study:

  • To investigate the functional link between S. pombe Ddb1 and the replication checkpoint control gene cds1.
  • To elucidate the role of Ddb1 in DNA replication and cell cycle regulation.

Main Methods:

  • Analysis of S. pombe strains lacking ddb1, including growth rate assessment and flow cytometry.
  • Phenotypic characterization of Deltaddb1 strains under UV irradiation and hydroxyurea treatment.
  • Measurement of Cds1 kinase activity in Deltaddb1 cells and analysis of cds1 mutations.

Main Results:

  • S. pombe lacking ddb1 exhibits slow growth due to delayed replication progression and DNA content heterogeneity.
  • Deltaddb1 strains are hypersensitive to UV irradiation and hydroxyurea, indicating replication stress sensitivity.
  • Deltaddb1 cells show elevated Cds1 kinase activity during S phase, and cds1 mutations rescue Deltaddb1 defects.

Conclusions:

  • S. pombe Ddb1 is functionally linked to Cds1, a key regulator of the replication checkpoint.
  • Defects in Deltaddb1 cells are largely attributed to aberrant Cds1 activation, highlighting Ddb1's role in regulating this pathway.