Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

NF-kappaB inhibits gammaherpesvirus lytic replication.

Helen J Brown1, Moon Jung Song, Hongyu Deng

  • 1Department of Molecular and Medical Pharmacology, University of California at Los Angeles, Los Angeles, California 90095, USA.

Journal of Virology
|July 15, 2003
PubMed
Summary

The cellular transcription factor NF-kappaB regulates gammaherpesvirus latency. High NF-kappaB activity inhibits lytic replication, promoting viral persistence in lymphocytes and offering a potential therapeutic target for associated cancers.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

G6PD inhibition prevents abdominal aortic aneurysm formation in mice induced by Ang II plus high salt.

Atherosclerosis·2026
Same author

RNF213 isoform 2 restricts Zika virus through antiviral signaling and viral protein degradation.

iScience·2026
Same author

Single cell and single nucleus RNA sequencing in liver tissues: applications and prospects in model and non-model organisms.

Frontiers in genetics·2026
Same author

Human obese gut microbiota induces lipid metabolic disorder and hypophagia without weight gain in normocaloric mice.

Science China. Life sciences·2026
Same author

A novel small-molecule inhibitor GSK-F1 confers radiosensitivity by inhibiting the NSUN2/TP53/RAD51 axis-mediated DNA homologous recombination repair in nasopharyngeal carcinoma.

International journal of biological sciences·2026
Same author

Antagonistic modulation of myosin conformational dynamics by sanshool and smoke-derived aldehydes: A molecular basis for heterocyclic amine control in thermal processing.

Food chemistry·2026

Area of Science:

  • Virology
  • Molecular Biology
  • Immunology

Background:

  • Gammaherpesviruses cause human malignancies like Kaposi's sarcoma.
  • These viruses establish lifelong latent infections in lymphocytes.
  • Viral latency involves minimal gene expression, evading immune detection.

Purpose of the Study:

  • Identify cell-specific factors controlling gammaherpesvirus replication-to-latency switch.
  • Investigate the role of the transcription factor NF-kappaB in this process.

Main Methods:

  • Overexpression of NF-kappaB in epithelial cells and fibroblasts.
  • Assessing the impact of NF-kappaB on murine herpesvirus 68 (MHV68) replication.
  • Analyzing NF-kappaB's effect on lytic promoters of MHV68, KSHV, and EBV.

Related Experiment Videos

  • Treating latently infected lymphocytes with an NF-kappaB inhibitor.

    • Main Results:

    • Overexpressing NF-kappaB inhibited MHV68 replication in non-lymphoid cells.
    • Elevated NF-kappaB suppressed lytic promoters of MHV68, KSHV, and EBV.
    • NF-kappaB inhibition reactivated latent KSHV and EBV, inducing lytic protein synthesis.

    Conclusions:

    • High NF-kappaB activity inhibits gammaherpesvirus lytic replication.
    • NF-kappaB likely contributes to establishing and maintaining viral latency in lymphocytes.
    • NF-kappaB represents a potential therapeutic target for disrupting gammaherpesvirus latency and treating associated cancers.