Related Experiment Videos
[Preparation and identification of rabbit-anti-human nestin antiserum]
Lei Liu1, Wan Min Lin, Jun Ji Lin
1Laboratory of Genomic Physiology, Brain Research Center, LiRen Laboratory, Fudan University, Shanghai 200433.
Shi Yan Sheng Wu Xue Bao
|July 16, 2003
Abstract:
Nestin belongs to the class VI intermediate filament family and it is a marker for neural progenitor cells. In this work, the 3'-terminal coding sequence(396 bp) of human nestin gene was cloned into pGEX-3X plasmid and introduced into BL21 E. coli cells. The GST-nestin protein was purified with an affinity column. Anti-human nestin antiserum was raised by immunizing a rabbit with the fusion protein. The high specificity of the antibody against human nestin was confirmed by western-blot and immunocytochemistry analysis.