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Updated: Aug 8, 2026

Drug-induced Sensitization of Adenylyl Cyclase: Assay Streamlining and Miniaturization for Small Molecule and siRNA Screening Applications
Published on: January 27, 2014
Modulation of adenylate cyclase signalling
1Laboratory of Tumor Immunology and Biology, National Cancer Institute, Bethesda, MD 20892.
Abstract:
Adenylate cyclase via production of cyclic AMP (cAMP) can transduce dual signals, positive and negative, on cell growth and differentiation. Such dual signals are transmitted by RI and RII cAMP-binding receptor proteins respectively, the regulatory subunits of cAMP dependent protein kinases. The growth stimulatory RI and inhibitory RII are in a strict balance to maintain normal cells, and departure from such balance can cause a great variety of human diseases, including cancer. Experimental approaches using site-selective cAMP analogues, antisense oligodeoxynucleotides, and gene transfer have shown that restoration of the normal balance of RI/RII provides a biological means to the suppression of malignancy.
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