[14C]-glucose metabolism of Oncomelania snails

To elucidate the importance of glucose metabolism in Oncomelania snails, uniformly labelled D (14C)-glucose [(U-14C)-glucose] has been used as a tracer for observing glucose uptake, incorporation into macromolecules and the activity of enzymes involved in its metabolic pathway. Radioactivity of Oncomelania increased with time of exposure (3, 6, 12 and 24 h) to [(U-14C)-glucose] solution. In snails treated with 2 ppm bromoacetamide for 24 h, the radioactivity of soft tissues was markedly less than that of normal snails. Oncomelania can synthesize glycogen from absorbed glucose, and the absorbed (U-14C)-glucose may be incorporated into nucleic acid, protein and lipid, with 14CO2 excreted as an end metabolic product. 6-phosphoglucose dehydrogenase and 6-phosphogluconate dehydrogenase activities were demonstrated by the reduction of NADP and determination of 14CO2 from (1-14C)-glucose, respectively. The results indicate that there exists a phosphopentose pathway in Oncomelania.