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Ultrastructural changes in stored platelets.

J G White1

  • 1Department of Laboratory Medicine and Pathology, University of Minnesota Medical School, Minneapolis 55455.

Blood Cells
|January 1, 1992
PubMed
Summary
This summary is machine-generated.

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Platelet storage lesions have decreased with improved blood bag technology. However, giant alpha granules persist, with their formation mechanism remaining unclear.

Area of Science:

  • Hematology
  • Blood banking
  • Cellular biology

Background:

  • Platelets stored in vitro develop lesions due to factors like poor gas exchange and low pH.
  • Advances in blood bag technology have mitigated some storage-induced platelet damage.

Purpose of the Study:

  • To investigate persistent storage lesions in blood platelets despite improved storage conditions.
  • To characterize the morphology and prevalence of specific platelet lesions over time.

Main Methods:

  • In vitro storage of platelets in contemporary blood bags.
  • Morphological analysis of platelets using microscopy.
  • Assessment of lesion frequency over a 14-day storage period.

Main Results:

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  • Modern blood bags reduce lesions associated with poor gas exchange and acidic pH.
  • Doughnut-shaped platelets appear early but are infrequent in current bags.
  • Giant alpha granules, formed by organelle fusion, are observed frequently from day 4-5 up to 14 days.

    • Conclusions:

    • While many platelet storage lesions are resolved by modern blood bags, giant alpha granule formation persists.
    • The underlying mechanism for giant alpha granule development in stored platelets requires further investigation.