Automated sequencing batch bioreactor under extreme peaks of 4-chlorophenol

The operation of a sequencing batch bioreactor is evaluated when high concentration peaks of a toxic compound (4-chlorophenol, 4CP) are introduced into the reactor. A control strategy based on the dissolved oxygen concentration, measured on line, is utilized. To detect the end of the reaction period, the automated system search for the moment when the dissolved oxygen has passed by a minimum, as a consequence of the metabolic activity of the microorganisms and right after to a maximum due to the saturation of the water (similar to the self-cycling fermentation, SCF, strategy). The dissolved oxygen signal was sent to a personal computer via data acquisition and control using MATLAB and the SIMULINK package. The system operating under the automated strategy presented a stable operation when the acclimated microorganisms (to an initial concentration of 350 mg 4CP/L), were exposed to a punctual concentration peaks of 600 mg 4CP/L. The 4CP concentrations peaks superior or equals to 1,050 mg/L only disturbed the system from a short to a medium term (one month). The 1,400 mg/L peak caused a shutdown in the metabolic activity of the microorganisms that led to the reactor failure. The biomass acclimated with the SCF strategy can partially support the variations of the toxic influent since, at the moment in which the influent become inhibitory, there is a failure of the system.