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Immunoglobulin light chains modulate polymorphonuclear leucocyte apoptosis

G Cohen1, M Rudnicki, R Deicher

  • 1Department of Medicine III, University of Vienna, Vienna, Austria. cohen@nephro.imed3.akh-wein.ac.at

European Journal of Clinical Investigation
|July 17, 2003
PubMed
Abstract

Insights

Immunoglobulin light chains (IgLCs) in uremic patients inhibit polymorphonuclear leucocyte (PMNL) apoptosis. This finding suggests IgLCs may hinder inflammation resolution and contribute to chronic inflammation in end-stage renal disease.

Area of Science:

  • Immunology
  • Cell Biology
  • Renal Medicine

Background:

  • Polymorphonuclear leucocyte (PMNL) apoptosis is crucial for resolving inflammation.
  • Previous research showed glucose-modified proteins enhance PMNL apoptosis.
  • No serum factors in uremic patients inhibiting PMNL apoptosis were previously identified.

Purpose of the Study:

  • To investigate the effect of immunoglobulin light chains (IgLCs) on PMNL apoptosis.
  • To determine if IgLCs found in uremic patients' sera influence PMNL apoptosis.

Main Methods:

  • Tested monoclonal and polyclonal IgLCs on PMNL apoptosis.
  • Assessed morphological changes, DNA strand breaks, and DNA content loss.
  • Investigated the role of tyrosine phosphorylation and caspase-3 inhibition.

Main Results:

  • Both kappa and lambda IgLCs inhibited PMNL apoptosis in a concentration-dependent manner.
  • IgLCs' effect was blocked by specific antibodies and genistein, indicating tyrosine phosphorylation involvement.
  • Inhibition of caspase-3 activity was linked to reduced PMNL apoptosis.

Conclusions:

  • IgLCs present in uremic patient sera can inhibit PMNL apoptosis.
  • This inhibition may disrupt normal inflammation resolution.
  • IgLCs could contribute to the chronic inflammatory state in end-stage renal disease.

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