Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Modelling intracellular H(+) ion diffusion.

Pawel Swietach1, Massimiliano Zaniboni, Andrew K Stewart

  • 1Burdon Sanderson Cardiac Science Centre, University Laboratory of Physiology, OX1 3PT, Oxford, UK

Progress in Biophysics and Molecular Biology
|July 17, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Age-related changes in lysosomal abundance in mouse hearts assessed by Lysotracker fluorescence imaging and autophagy gene expression analysis.

The international journal of cardiovascular imaging·2026
Same author

A reproducible and physiologically relevant human iPSC-derived platform for in vitro modeling of the neurocardiac junction.

Molecular therapy : the journal of the American Society of Gene Therapy·2026
Same author

The electrophysiology of pairs of cardiac atrial and pacemaker cells.

Progress in biophysics and molecular biology·2026
Same author

pH-Dependent Microenvironmental Ionic Signaling in Pancreatic Ductal Adenocarcinoma.

Acta physiologica (Oxford, England)·2026
Same author

Relief with a catch: hemodilution in chronic mountain sickness sparks an erythropoietin surge.

Blood. Red cells & iron·2026
Same author

Urinary carbonic anhydrase 1 excretion is a marker of hemolysis-triggering conditions suitable for point-of-care testing.

Blood global hematology·2026

Computational models reveal how intracellular pH regulation is affected by proton diffusion, membrane transport, and cell shape. This research provides a framework for understanding pH non-uniformity in cells.

Area of Science:

  • Cellular Physiology
  • Computational Biology
  • Biophysics

Background:

  • Intracellular pH (pH(i)) is crucial for cell function and regulated by membrane transport and intracellular buffering systems.
  • Proton (H+) mobility is limited by buffers, influencing the spatial distribution of pH(i).
  • Understanding pH(i) dynamics is vital for cellular homeostasis.

Purpose of the Study:

  • To analyze the spatio-temporal behavior of intracellular [H+] using computational methods.
  • To investigate the impact of membrane transport and cell geometry on pH(i) distribution.
  • To develop a mechanistic model for pH(i) regulation in cardiac myocytes.

Main Methods:

  • Utilized finite difference (FDM) and finite element methods (FEM) for computational modeling.

Related Experiment Videos

  • Analyzed experimental confocal imaging data of pH(i) in various cell types.
  • Developed mathematical algorithms to determine apparent intracellular H+ diffusion coefficients (D(H)(app)).

    • Main Results:

    • Computational models successfully fitted experimental data for pH(i) imaging.
    • Explored the influence of membrane transport and cell geometry on [H+] distribution.
    • Developed a mechanistic model for pH(i) changes in cardiac myocytes, incorporating buffer shuttling and anchoring.

    Conclusions:

    • Computational approaches provide a robust framework for analyzing intracellular pH dynamics.
    • The study highlights the importance of considering proton diffusion, buffering, and cell structure in pH(i) regulation.
    • This work lays the foundation for future research on the physiological consequences of pH(i) non-uniformity.