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Catabolite repression in Enterococcus faecalis.

Mary C Rea1, Timothy M Cogan

  • 1Dairy Products Research Centre, Teagasc, Fermoy, Co. Cork, Ireland.

Systematic and Applied Microbiology
|July 18, 2003
PubMed
Summary
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Enterococcus faecalis utilizes citrate and pyruvate as energy sources. Glucose and fructose trigger catabolite repression, inhibiting citrate metabolism in these bacteria.

Area of Science:

  • Microbiology
  • Bacterial Metabolism
  • Molecular Biology

Background:

  • Enterococcus faecalis is a common bacterium with diverse metabolic capabilities.
  • Understanding substrate utilization is crucial for comprehending bacterial growth and adaptation.
  • Catabolite repression is a key regulatory mechanism influencing microbial metabolism.

Purpose of the Study:

  • To investigate the metabolism of citrate, pyruvate, and sugars by Enterococcus faecalis strains.
  • To elucidate the effects of glucose and fructose on citrate and pyruvate utilization.
  • To characterize the role of catabolite repression in Enterococcus faecalis.

Main Methods:

  • Growth rate determination of Enterococcus faecalis E-239 and JH2-2 on various carbon sources.

Related Experiment Videos

  • Analysis of citrate and pyruvate metabolism in the presence of sugars.
  • Comparison of wild-type strains with a catabolite derepressed mutant (CL4).

    • Main Results:

    • Enterococcus faecalis utilizes citrate and pyruvate as energy sources.
    • Glucose and fructose inhibit citrate metabolism, demonstrating catabolite repression.
    • A catabolite derepressed mutant showed co-metabolism of citrate and glucose, unlike the wild-type.
    • The degree of citrate metabolism inhibition correlated with glucose concentration.

    Conclusions:

    • Catabolite repression by glucose and fructose is a significant regulatory mechanism in Enterococcus faecalis.
    • This repression impacts the utilization of alternative carbon sources like citrate.
    • The findings provide insights into the metabolic flexibility and regulation in enterococci.