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Alterations in phage-typing patterns in vancomycin-intermediate Staphylococcus aureus.

John E Gustafson1, Frances G O'Brien1, Geoffrey W Coombs1

  • 1Department of Biology, New Mexico State University, Las Cruces, NM 88003-8001, USA 2Gram-positive Bacteria Typing and Research Unit, Curtin University of Technology, and Royal Perth Hospital, Perth 6845, Western Australia 3Microbiology Group, Department of Biological Sciences, Illinois State University, Normal, IL 61790-4120, USA.

Journal of Medical Microbiology
|July 18, 2003
PubMed
Summary
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Phage-typing and SmaI RFLPs were used to analyze vancomycin-intermediate Staphylococcus aureus (VISA) strains. SmaI RFLPs showed strain relatedness, but phage-typing did not, indicating potential issues in epidemiological studies.

Area of Science:

  • Microbiology
  • Molecular Biology
  • Epidemiology

Background:

  • Vancomycin-intermediate Staphylococcus aureus (VISA) poses a significant challenge in healthcare settings.
  • Accurate strain typing is crucial for understanding the epidemiology of antibiotic-resistant bacteria.

Purpose of the Study:

  • To evaluate the utility of phage-typing and SmaI chromosomal RFLPs for assessing strain relatedness between VISA and their susceptible parent strains.
  • To determine if these methods accurately reflect clonal relationships in vitro-selected VISA.

Main Methods:

  • Analysis of 12 vancomycin-intermediate Staphylococcus aureus (VISA) strains and their 7 vancomycin-susceptible parent strains.
  • Application of phage-typing and SmaI chromosomal Restriction Fragment Length Polymorphism (RFLP) analysis.

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Main Results:

  • SmaI RFLP analysis revealed clear clonal relationships between isogenic parent and VISA strains, with generally no observed alterations.
  • Phage-typing profiles frequently differed between parent and VISA strains, with some VISA strains becoming untypable.
  • Observed discrepancies suggest cell wall alterations in VISA strains affecting phage receptor sites.

Conclusions:

  • SmaI RFLP is a reliable method for determining strain relatedness between isogenic VISA and susceptible Staphylococcus aureus.
  • Phage-typing alone may lead to inaccurate epidemiological conclusions due to VISA-induced alterations.
  • Combined use of genotypic and phenotypic methods is recommended for accurate epidemiological assessments of VISA strains.