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Updated: Aug 11, 2026

Robust 3D DNA FISH Using Directly Labeled Probes
Published on: August 15, 2013
DNA-Naphthyl Red conjugate as a visualizing probe of DNA hybridization
Hiroyuki Asanuma1, Hiromu Kashida, Xingguo Liang
1Research Center for Advanced Science and Technology, The University of Tokyo, Komaba, Meguro-ku, Tokyo 153-8904, Japan. asanuma@mkomi.rcast.u-tokyo.ac.jp
Abstract:
The Naphthyl Red moiety, conjugated to DNA, shows distinct chromism by hybridization with its complementary DNA. Single-stranded DNA involving the Naphthyl Red moiety exhibits an orange color and has lambda(max) at 466 nm at pH 7.0. The absorption maximum is shifted towards 545 nm by the presence of its complementary DNA, and the color of the solution changes from orange to magenta accordingly.
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