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Related Experiment Videos

Afterpulsing and its correction in fluorescence correlation spectroscopy experiments.

Ming Zhao1, Lei Jin, Bo Chen

  • 1Laboratory of Atomic, Molecular and Nanosciences, Tsinghua University, Beijing 100084, China.

Applied Optics
|July 19, 2003
PubMed
Summary
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Photon detector afterpulsing, a common issue in photon correlation experiments, can be corrected. This study demonstrates afterpulsing and presents a software solution to eliminate it in autocorrelation measurements.

Area of Science:

  • Photonics
  • Spectroscopy
  • Experimental Physics

Background:

  • Afterpulsing in photon detectors is caused by feedback mechanisms.
  • This phenomenon generates spurious signal pulses following true detection events.
  • Afterpulsing significantly complicates photon correlation spectroscopy.

Purpose of the Study:

  • To demonstrate the afterpulsing effect in fluorescence correlation spectroscopy (FCS).
  • To develop and validate a theoretical and software-based method for correcting afterpulsing in FCS.
  • To compare the corrected autocorrelation results with cross-correlation measurements.

Main Methods:

  • Utilized different avalanche photodiodes to exhibit the afterpulsing effect.
  • Developed a theoretical framework to eliminate leading-order afterpulsing in autocorrelation.

Related Experiment Videos

  • Implemented software for automated afterpulsing correction.
  • Performed comparative analysis with cross-correlation techniques.

    • Main Results:

    • Successfully demonstrated afterpulsing in avalanche photodiodes during FCS.
    • The developed theoretical approach effectively eliminates leading-order afterpulsing.
    • Software implementation proved straightforward and efficient for correction.
    • Comparison with cross-correlation validated the accuracy of the corrected autocorrelation.

    Conclusions:

    • Afterpulsing in photon detectors is a significant challenge in photon correlation experiments.
    • A robust theoretical and computational method is presented to correct for afterpulsing in autocorrelation.
    • The findings facilitate more accurate fluorescence correlation spectroscopy measurements.