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Whole-field fluorescence microscope with digital micromirror device: imaging of biological samples
Takashi Fukano1, Atsushi Miyawaki
1Laboratory for Cell Function and Dynamics, Advanced Technology Development Group, Institute of Brain Science, The Institute of Physical and Chemical Research, 2-1 Hirosawa, Wako-city, Saitama 351-0198, Japan. tenchan@brain.riken.go.jp
Abstract:
We have developed a whole-field fluorescence microscope equipped with a Digital Micromirror Device to acquire optically sectioned images by using the fringe-projection technique and the phase-shift method. This system allows free control of optical sectioning strength through computer-controlled alteration of the fringe period projected onto a sample. We have employed this system to image viable cells expressing fluorescent proteins and discussed its biological applications.