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Related Experiment Videos

Polymorphisms in human soluble epoxide hydrolase.

Beata D Przybyla-Zawislak1, Punit K Srivastava, Johana Vazquez-Matias

  • 1Department of Pharmaceutical Sciences, University of Connecticut, 372 Fairfield Road, Unit 2092, Storrs, CT 06269-2092, USA.

Molecular Pharmacology
|July 19, 2003
PubMed
Summary

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Human soluble epoxide hydrolase (hsEH) gene variants impact enzyme function. Six identified polymorphisms, including four with functional consequences, influence epoxide metabolism in humans.

Area of Science:

  • Biochemistry
  • Human Genetics
  • Enzymology

Background:

  • Human soluble epoxide hydrolase (hsEH) metabolizes epoxides to vicinal diols.
  • Endogenous substrates include arachidonic and linoleic acid epoxides.
  • Significant interindividual variation in hsEH activity suggests genetic polymorphisms.

Purpose of the Study:

  • To identify and characterize genetic polymorphisms in the human soluble epoxide hydrolase (hsEH) gene (EPHX2).
  • To assess the functional impact of identified hsEH variants on enzyme activity and substrate metabolism.

Main Methods:

  • Resequencing of all 19 exons of the EPHX2 gene in diverse populations.
  • Site-directed mutagenesis to create hsEH variants.
  • Expression of mutant hsEH in insect cells and enzymatic activity assays.

Related Experiment Videos

  • Kinetic studies (Km, Vmax) and protein stability assessments.

    • Main Results:

    • Six hsEH polymorphisms were identified, with six resulting in amino acid substitutions.
    • Arg287Gln and a double mutant (Arg287Gln/Arg103Cys) showed decreased enzyme activity.
    • Lys55Arg and Cys154Tyr mutants exhibited elevated activity.
    • The double mutant displayed altered kinetics (Km, Vmax) and reduced stability.

    Conclusions:

    • At least six functional hsEH variants exist in the human population.
    • Four identified variants may significantly influence the in vivo metabolism of epoxide substrates.
    • These findings contribute to understanding human epoxide metabolism variability.