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Updated: Sep 23, 2026

A Restriction Enzyme Based Cloning Method to Assess the In vitro Replication Capacity of HIV-1 Subtype C Gag-MJ4 Chimeric Viruses
Published on: August 31, 2014
HIV and gamete interactions
1Assisted Conception Unit, 4th Floor, Thomas Guy House, Guy's Hospital, London SE1 9RT, UK.
Abstract:
With the development of combination therapies and their use for the treatment of the human immunodeficiency virus (HIV), the health and life expectancy of HIV-positive patients has improved significantly. As a result, HIV-discordant couples are looking to a future that involves starting a family. However, the potential risks of exposing HIV-infected sperm to oocytes are still being assessed. This article outlines the clinical detection of HIV in semen samples, the relationships between HIV and semen parameters, and HIV-sperm and oocyte interactions. It also considers the implications of introducing exogenous genetic material into an oocyte via intracytoplasmic sperm injection (ICSI) and the possible implications of this for assisted reproduction.
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