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[In vitro infection of human liver cancer cell line HepG2 with HCV]
Zhi-qiang Song1, Fei Hao, Qiao-yu Ma
1Center for Infectious Disease of PLA, Southwest Hospital, Third Military Medical University, Chongqing 400038, China.
Background:
To test susceptibility of human liver cell line Hep G2 to HCV in vitro.
Methods:
Hep G2 was cultivated with the serum from a chronic hepatitis C patient. After inoculation, plus and minus strand of HCV RNA, the expression of HCV NS3 antigen and the location of HCV RNA in cell and/or supernatant were examined by RT-PCR, immunohistochemistry and in situ hybridization, respectively.
Results:
HCV RNA could be detected from day 2 to day 40 post-inoculation in both cell and supernatant. HCV NS3 antigen could be expressed in infected cells and HCV RNA was mainly situated within cytoplasm.
Conclusions:
The results suggested that HepG2 cell line was not only susceptible to HCV but also could support its replication in vitro.