Site-directed mutagenesis and preliminary x-ray crystallographic studies of the tabtoxin resistance protein
Yi Ding1, Shentao Li, Xiaofeng Li
1Laboratory of Structural Biology, School of Life Science & Engineering, Tsinghua University, Beijing 100084, China.
Protein and Peptide Letters
|July 23, 2003
Abstract:
Tabtoxin resistance protein (TTR) is an enzyme that catalyzes the acetylation of tabtoxin rendering tabtoxin-producing pathogens tolerant to their own phytotoxins. According to the structure based detoxification mechanism of TTR, three site-directed mutants Y141F, D130N and Y141F-D130N were constructed and overexpressed in E. coli. The products were then purified and their properties were analyzed by CD and DLS. Crystallization trials of two mutants Y141F andY141F-D130N were preformed.
