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Oxidation of goat hepatic galectin-1 induces change in secondary structure
Abhay H Pande1, Rajesh K Gupta, Sumati
1School of Life Sciences, Devi Ahilya Vishwavidyalaya, Vigyan Bhawan, Khandwa Road Campus, Indore 452 001 (MP), India.
Protein and Peptide Letters
|July 23, 2003
Abstract:
Galectin-1 requires a reducing environment for its lectin activity and the carbohydrate binding function is destroyed in oxidizing condition. In this report we provide direct evidence that the oxidation of goat hepatic galectin-1 perturbs its carbohydrate recognition domain and this could be due to changes in secondary structure of goat hepatic galectin-1. Conformational changes in goat hepatic galectin-1 due to oxidation were investigated by absorption, fluorescence and circular dichroism measurements.