Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Eosinophil IgE receptor and CD23.

M Capron1, M J Truong, D Aldebert

  • 1Centre d'Immunologie et de Biologie Parasitaire, Unité Mixte INSERM U167-CNRS 624, Institut Pasteur, Lille, France.

Immunologic Research
|January 1, 1992
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The interleukin 2 receptor in the hypereosinophilic syndrome.

Leukemia & lymphoma·1992
Same author

Protection of mice and nude rats against toxoplasmosis by a multiple antigenic peptide construction derived from Toxoplasma gondii P30 antigen.

Journal of immunology (Baltimore, Md. : 1950)·1992
Same author

Determination of B-cell epitopes of nef HIV-I protein: immunogenicity related to their structure.

Molecular immunology·1992
Same author

Amino acid sequence requirements for the epitope recognized by a monoclonal antibody reacting with the secreted antigen GP28.5 of Toxoplasma gondii.

Molecular immunology·1992
Same author

Fibronectin cleavage fragments provide a growth factor-like activity for the differentiation of Trypanosoma cruzi trypomastigotes to amastigotes.

European journal of cell biology·1992
Same author

Antibodies in rheumatoid synovial fluids bind to a restricted series of protein antigens in rheumatoid synovial tissue.

Arthritis and rheumatism·1992
Same journal

Emapalumab plus conventional therapy with or without ruxolitinib for pediatric hemophagocytic lymphohistiocytosis: a single center retrospective study.

Immunologic research·2026
Same journal

Immunological spectrum in patients with thymoma: beyond good syndrome.

Immunologic research·2026
Same journal

Microbiome immune crosstalk in Sjögren's syndrome: mechanistic insights and translational perspectives.

Immunologic research·2026
Same journal

Immune checkpoint inhibitor-induced myasthenia gravis and myocarditis: a fatal immune-related adverse event.

Immunologic research·2026
Same journal

TRIM28 and TRIM32: multifaceted regulators of innate immunity and antiviral defence.

Immunologic research·2026
Same journal

Decoding PANoptosis: Crosstalk of cell death pathways in immunity and inflammation.

Immunologic research·2026
See all related articles

Eosinophil Fc epsilon RII shares similarities with the B cell marker CD23, playing a role in IgE-dependent eosinophil functions. However, differences in expression and effects suggest distinct molecular characteristics.

Area of Science:

  • Immunology
  • Cell Biology

Background:

  • Eosinophils and B cells are key immune cells involved in allergic responses.
  • CD23 is a well-established differentiation marker for B cells, also known as Fc epsilon RII.
  • The precise role and nature of Fc epsilon RII on eosinophils have been areas of investigation.

Purpose of the Study:

  • To compare eosinophil Fc epsilon RII with the B cell differentiation marker CD23.
  • To investigate the functional implications of CD23 or related molecules on eosinophils in IgE-dependent processes.

Main Methods:

  • Biochemical analysis including immunoprecipitation was used to compare molecules from eosinophils and B cells.
  • Flow cytometry was employed to assess the correlation between anti-CD23 monoclonal antibody (mAb) binding and IgE.
  • Northern blot analysis was performed on eosinophil RNA to detect CD23 gene expression.

Related Experiment Videos

Main Results:

  • Similar molecular weights of Fc epsilon RII were immunoprecipitated from eosinophils and B cells using anti-CD23 or anti-Fc epsilon RII antibodies.
  • A correlation between anti-CD23 mAb binding and IgE was observed via flow cytometry.
  • Low CD23 expression and weak messenger RNA signals were found in hypereosinophilic patients, despite functional inhibition of IgE-mediated eosinophil functions by anti-CD23 mAbs.

Conclusions:

  • CD23 or a related molecule is involved in IgE-dependent eosinophil functions, including IgE binding and cytotoxicity.
  • Despite similarities, differential effects of anti-CD23 mAbs on eosinophils and B cells indicate distinct molecular characteristics of CD23 expressed on these cell types.