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Resveratrol and quercetin down-regulate tissue factor expression by human stimulated vascular cells
A Di Santo1, A Mezzetti, E Napoleone
1Antonio Taticchi Unit for Atherosclerosis and Thrombosis, Istituto Ricerche Farmacologiche Mario Negri, Consorzio Mario Negri Sud, S. Maria Imbaro, Italy.
Journal of Thrombosis and Haemostasis : JTH
|July 23, 2003
Summary
Resveratrol and quercetin, found in red wine, significantly reduce tissue factor (TF) expression in endothelial and mononuclear cells. This molecular mechanism may explain red wine's cardioprotective effects against cardiovascular disease.
Area of Science:
- Cardiovascular Research
- Molecular Biology
- Nutritional Science
Background:
- Epidemiological studies link red wine consumption to reduced coronary heart disease risk.
- Resveratrol and quercetin, polyphenols in grapes, exhibit cardioprotective biological properties.
- Tissue factor (TF) initiates coagulation and is implicated in atherosclerosis and thrombosis.
Purpose of the Study:
- Investigate the effects of resveratrol and quercetin on TF expression.
- Examine TF modulation in human endothelial and mononuclear cells.
Main Methods:
- Human umbilical vein endothelial cells and mononuclear cells were stimulated with agonists.
- Cells were pre-incubated with varying concentrations of resveratrol or quercetin.
- TF activity, mRNA levels, and nuclear factor binding were analyzed.
Main Results:
- Resveratrol and quercetin dose-dependently reduced TF activity and mRNA expression.
- Inhibition of TF mRNA correlated with reduced c-Rel/p65 nuclear binding.
- This effect was linked to the inhibition of IkappaBalpha degradation.
Conclusions:
- Resveratrol and quercetin suppress TF expression through molecular pathways.
- Findings provide a basis for red wine's cardiovascular protective effects.
- Highlights the role of TF regulation in wine-mediated cardioprotection.