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Simple and rapid methods for SEM observation and TEM immunolabeling of rubber particles
Adya P Singh1, Seung Gon Wi, Hunseung Kang
1Agricultural Plant Stress Research Center, Chonnam National University, Gwangju, Republic of Korea. adyasingh@hotmail.com
Abstract:
We developed a method involving air-drying of a rubber suspension after fixation in glutaraldehyde-tannic acid and postfixation in osmium tetroxide for SEM observation. For TEM immunolabeling the suspension was air-dried after osmium-only fixation. Whereas conventional methods failed to satisfactorily stabilize rubber particles, the methods described here proved successful in preserving their integrity.