Related Experiment Videos
Patched 1 conditional null allele in mice
Tammy Ellis1, Ian Smyth, Emily Riley
1Institute for Molecular Bioscience, University of Queensland, Brisbane Queensland, Australia.
Abstract:
The patched gene (Ptc) is a member of the hedgehog signaling pathway which plays a central role in the development of many invertebrate and vertebrate tissues. In addition, Ptc and a number of other pathway members are mutated in some common human cancers. Patched is the receptor for the hedgehog ligand and in the mouse ablation of the Ptc gene leads to developmental defects and an embryonic lethal phenotype. Here we describe a conditional Ptc allele in mice which will have utility for the temporospatial ablation of Ptc function.
Insights
Researchers developed a conditional mouse model to study the patched gene (Ptc), a key player in hedgehog signaling. This tool allows for precise control over Ptc gene function, aiding research into development and cancer.
Area of Science:
- Developmental Biology
- Molecular Biology
- Cancer Biology
Background:
- The patched gene (Ptc) is crucial for the hedgehog signaling pathway, essential in tissue development.
- Mutations in Ptc and other hedgehog pathway components are implicated in common human cancers.
- Ptc acts as the receptor for the hedgehog ligand, and its absence causes embryonic lethality in mice.
Purpose of the Study:
- To create a conditional Ptc allele in mice.
- To enable temporospatial ablation of Ptc function.
- To facilitate research on the role of Ptc in development and disease.
Main Methods:
- Generation of a conditional Ptc allele in mice.
- Utilizing genetic tools for targeted gene inactivation.
Main Results:
- A conditional Ptc allele was successfully created.
- This allele allows for controlled inactivation of Ptc function in specific tissues and at specific times.
Conclusions:
- The conditional Ptc allele provides a valuable tool for studying the gene's function.
- This model will advance research into the roles of hedgehog signaling in development and cancer.