Related Experiment Video
Updated: Sep 22, 2026
Soybean Hairy Root Transformation for the Analysis of Gene Function
Published on: May 5, 2023
Cloning and expression of the isoflavone synthase gene (IFS-Tp) from Trifolium pratense
Bong Gyu Kim1, Song-Young Kim, Hee Su Song
1Department of Forest and Environmental Science, Bio/Molecular Informatics Center, KonKuk University, Seoul 143-701, Korea.
Abstract:
Isoflavones are secondary metabolites found mainly in leguminous plants. Their synthesis from flavanones is catalyzed by isoflavone synthase (IFS). We have cloned a isoflavone synthase gene (IFS-Tp) from Trifolium pratense that encodes a predicted 525 amino acids protein, molecular weight 59 kDa, with strong homology to IFS's from other legumes. IFS-Tp was expressed in all the tissues examined, and addition of glutathione and UV irradiation enhanced its expression. Microsomes from yeast transformed with IFS-Tp were able to convert naringenin to genistein, indicating that IFS-Tp has isoflavone synthase activity.
Related Concept Videos
02:50Transgenic Plants
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
02:27Trihybrid Crosses
Some of Mendel’s crosses examined three pairs of contrasting characteristics. Such a cross is called a trihybrid cross. A trihybrid cross is a combination of three individual monohybrid crosses. For example, plant height (tall vs. short), seed shape (round vs. wrinkled), and seed color (yellow vs. green).
The F1 generation plants of a trihybrid cross are heterozygous for all three traits and produce eight gametes. Upon self-fertilization, these gametes have an equal chance to...