Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A novel, modification-dependent ATP-binding aptamer selected from an RNA library incorporating a cationic

Narendra K Vaish1, Rosa Larralde, Andrew W Fraley

  • 1Department of Chemistry, Boston College, 140 Commonwealth Avenue, Chestnut Hill, Massachusetts 02467, USA. vaishn@sirna.com

Biochemistry
|July 23, 2003
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A ribozyme ligase that requires a 3' terminal phosphate on its RNA substrate.

Nature communications·2026
Same author

A nucleic acid labeling chemistry reveals surface DNA on exosomes.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Ultraviolet-driven self-repair in chimeric d(GAUU) outcompetes damage formation.

Chemical communications (Cambridge, England)·2026
Same author

Mg<sup>2+</sup> Catalyzes Nonenzymatic RNA Primer Extension through a Concerted Outer-Sphere Mechanism.

Journal of the American Chemical Society·2026
Same author

Influence of Phosphate Activation Chemistry on the Selection of the Primordial Genetic Alphabet.

Journal of the American Chemical Society·2026
Same author

Evolution of error correction through a need for speed.

Science (New York, N.Y.)·2026

Researchers created a novel RNA receptor that binds ATP by incorporating a cationic functional group. This modified RNA shows unique interactions with ATP, offering insights into the RNA world and potential diagnostic tools.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Synthetic Biology

Background:

  • RNA molecules can be engineered to bind specific targets.
  • Functionalized nucleic acids offer new possibilities for molecular recognition.

Purpose of the Study:

  • To develop a novel RNA receptor capable of binding Adenosine Triphosphate (ATP).
  • To investigate the role of cationic functional groups in RNA-ligand interactions.
  • To explore implications for the RNA world hypothesis and potential applications.

Main Methods:

  • Enzymatic polymerization to incorporate a cationic uridine triphosphate analogue into an RNA library.
  • In vitro selection to isolate functional RNA sequences.
  • Sequence analysis and secondary structure modeling to determine the ATP binding site.

Related Experiment Videos

  • Mutational studies and analysis of ATP analogue binding.

    • Main Results:

    • A novel RNA receptor that binds ATP under physiological conditions was identified.
    • Binding is critically dependent on the incorporated cationic functional group.
    • The modified RNA receptor interacts extensively with the triphosphate moiety of ATP, unlike natural aptamers.
    • Several modified uridines were found to be essential for ATP binding.

    Conclusions:

    • Incorporating cationic groups into nucleic acids enables novel ligand interactions.
    • This approach has significant implications for understanding early life (RNA world hypothesis).
    • Functionalized nucleic acids represent promising materials for research and diagnostics.