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Chromosomal radiosensitivity: a study of the chromosomal G(2) assay in human blood lymphocytes indicating significant
V Smart1, G B Curwen, C A Whitehouse
1Genetics Unit, Westlakes Research Institute, Moor Row, CA24 3JY Cumbria, UK. valerie.smart@westlakes.ac.uk
Abstract:
The G(2) chromosomal radiosensitivity assay is a technically demanding assay. To ensure that it is reproducible in our laboratory, we have examined the effects of storage and culture conditions by applying the assay to a group of healthy controls and determined the extent of intra- and inter-individual variations. Nineteen different individuals provided one or more blood samples resulting in a total of 57 successful tests. Multiple cultures from a single blood sample showed no statistically significant difference in the number of chromatid type aberrations between cultures. A 24h delay prior to culturing the lymphocytes did not significantly affect the induced G(2) score. Intra-individual variation was not statistically significant in seven out of nine individuals. Inter-individual variation was highly statistically significant (P<0.001), indicating that there is a real difference between individuals in the response to radiation using this assay.
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