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Related Experiment Videos

Interaction between DMPC liposomes and HM-PNIPAM polymer.

Yan jun Wang1, Françoise M Winnik, Ronald J Clarke

  • 1School of Chemical Technology and Engineering, Tianjin University, 300072, Tianjin, PR China. yjwang64@yahoo.com.cn

Biophysical Chemistry
|July 25, 2003
PubMed
Summary

Hydrophobically-modified poly-(N-isopropylacrylamides) (HM-PNIPAM) interact with lipid vesicles, influencing dye diffusion. HM-PNIPAM increases vesicle membrane fluidity, affecting dye movement within the lipid bilayer.

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Corrigendum to "Interaction of N-terminal peptide analogues of the Na+,K+-ATPase with membranes" [BBA Biomembr. 1860 (6) (2018) 1282-1291].

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Area of Science:

  • Biophysics
  • Polymer Science
  • Membrane Biophysics

Background:

  • Lipid-polymer interactions are crucial for understanding membrane dynamics.
  • Hydrophobically-modified poly-(N-isopropylacrylamides) (HM-PNIPAM) are synthetic polymers with potential applications in drug delivery and biomaterials.
  • Dimyristoylphosphatidylcholine (DMPC) is a common phospholipid used to model cell membranes.

Purpose of the Study:

  • To investigate the interactions between HM-PNIPAM and DMPC vesicles.
  • To determine the effect of HM-PNIPAM on the binding and diffusion of oxonol VI dye across DMPC membranes.
  • To elucidate how HM-PNIPAM influences the fluidity of the DMPC vesicle membrane.

Main Methods:

  • Stopped-flow fluorescence spectrophotometry was used to monitor dye-vesicle interactions.

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  • The binding and diffusion of oxonol VI dye were analyzed by observing fluorescence changes.
  • Kinetic analysis, including relaxation time measurements, was employed to study the two-stage process.

    • Main Results:

    • Oxonol VI binding to DMPC vesicles occurred in a two-stage process: rapid dye binding and slower intra-membrane diffusion.
    • HM-PNIPAM slightly slowed the dissociation of oxonol VI from the DMPC membrane.
    • HM-PNIPAM significantly increased the rate of the slow stage, indicating enhanced membrane fluidity.

    Conclusions:

    • HM-PNIPAM alters the dynamics of lipid membranes.
    • The polymer's presence increases the fluidity of DMPC vesicles, facilitating faster dye movement within the membrane.
    • These findings contribute to understanding polymer-membrane interactions and their impact on membrane properties.