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Reactive oxygen species cause diabetes-induced decrease in renal oxygen tension

F Palm1, J Cederberg, P Hansell

  • 1Department of Medical Cell Biology, Biomedical Center, Box 571, 751 23 Uppsala, Sweden. Fredrik.Palm@medcellbiol.uu.se

Diabetologia
|July 25, 2003
PubMed
Abstract

Insights

Diabetic nephropathy involves increased oxidative stress and oxygen consumption in the kidney medulla. Vitamin E treatment mitigated these effects, but not renal hypertrophy or hyperfiltration.

Area of Science:

  • Nephrology
  • Endocrinology
  • Physiology

Background:

  • Hyperglycemia-induced reactive oxygen species (ROS) are implicated in diabetic nephropathy development.
  • Understanding the impact of diabetes on renal oxygen dynamics is crucial for therapeutic strategies.

Purpose of the Study:

  • To investigate the effects of streptozotocin-induced diabetes on regional renal blood flow, oxygen tension, and consumption in rats.
  • To evaluate the protective role of alpha-tocopherol (vitamin E) in preventing diabetes-related oxidative stress and renal oxygen disturbances.

Main Methods:

  • Utilized streptozotocin-induced diabetes model in Wistar Furth rats.
  • Measured regional renal blood flow using laser-Doppler and oxygen tension/consumption with microelectrodes.
  • Administered DL-alpha-tocopherol (vitamin E) to assess its impact on oxidative stress markers and oxygen dynamics.

Main Results:

  • Diabetic rats exhibited reduced renal oxygen tension, particularly in the medulla, and increased oxygen consumption.
  • Diabetes elevated lipid peroxidation and protein carbonylation in the renal medulla.
  • Alpha-tocopherol treatment normalized oxygen tension and consumption, and reduced oxidative stress markers.
  • Renal hypertrophy and glomerular hyperfiltration in diabetic rats were not affected by alpha-tocopherol.

Conclusions:

  • Oxidative stress in diabetic kidneys, concentrated in the medulla, is linked to increased oxygen consumption and reduced oxygen tension.
  • Alpha-tocopherol effectively counteracts diabetes-induced oxidative stress and oxygen dysregulation in the kidney.
  • While vitamin E addresses oxidative aspects, it does not impact structural changes like hypertrophy or functional changes like hyperfiltration.

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