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Related Experiment Videos

B1 insertions as easy markers for mouse population studies.

Pavel Munclinger1, Pierre Boursot, Barbara Dod

  • 1Biodiversity Research Group, Department of Zoology, Faculty of Science, Charles University, Vinicná 7, CZ 128 44 Praha 2, Czech Republic. muncling@natur.cuni.cz

Mammalian Genome : Official Journal of the International Mammalian Genome Society
|July 25, 2003
PubMed
Summary

Researchers identified B1 short interspersed DNA elements (SINEs) as valuable genetic markers for wild mouse populations. These markers reveal genetic variation within Mus musculus subspecies and related species.

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Area of Science:

  • Genetics
  • Molecular Biology
  • Evolutionary Biology

Background:

  • Limited availability of simple PCR markers for genetic variation studies in wild Mus musculus populations.
  • Short interspersed DNA elements (SINEs) represent a potential source for novel genetic markers.

Purpose of the Study:

  • To evaluate the B1 family of SINEs as a source of polymorphic markers for population and subspecific genetic studies in mice.
  • To investigate genetic variation within and between different Mus species and subspecies using B1 elements.

Main Methods:

  • Retrieved thirteen B1 sequences based on homology to a mouse consensus sequence.
  • Screened for B1 element presence and insertion polymorphism in wild-derived mice (M. spretus, M. macedonicus, M. spicilegus, and M. musculus subspecies).

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  • Analyzed restriction site polymorphism at six B1 loci within M. musculus and related species.

    • Main Results:

    • Insertion polymorphism at five B1 loci was primarily found in Mus musculus domesticus, largely absent in other subspecies and species.
    • Six B1 elements were fixed across tested Mus species, but showed restriction site polymorphism within M. musculus.
    • Polymorphisms at these loci were often ancestral, shared with other Mus species.
    • Both insertion and restriction polymorphisms differentiated inbred laboratory strains, with some strains showing unexpected frequencies of Far Eastern alleles.

    Conclusions:

    • B1 SINEs are a valuable and abundant source of polymorphic markers for population genetics in wild mice.
    • B1 markers effectively distinguish between Mus species and subspecies, and reveal genetic diversity within M. musculus.
    • Laboratory mouse strains exhibit complex genetic origins, with some loci suggesting a higher prevalence of Far Eastern alleles than anticipated.